multiplex pcr study of plasmid-mediated ampc beta-lactamase genes in clinical isolates of escherichia coli
نویسندگان
چکیده
background : ampc β-lactamases are important cephalosporinases chromosomally encoded in many of enterobacteriaceae and a few other organisms where they mediate resistance to cephalothin, cefazolin, cefoxitin and penicillins. the six different families of plasmid-mediated ampc β-lactamases have been described, but no phenotypic test can discriminate among them. ampc multiplex pcr has been successfully used to discriminate plasmid-mediated ampc specific families in organisms such as klebsiella pneumonia and escherichia coli . the aim of this study was to indicate the prevalence of ampc β-lactamase genes by specifically designed primers through pcr test. methods : 243 total clinical urine samples were collected, and 227 isolates were identified as escherichia coli based on standard biochemical tests. subsequently, the isolates were screened by disc diffusion and combined disc test for β-lactamase production. resistant isolates were evaluated by pcr for ampc family determination. results : antibiotic resistance pattern were observed as follows: cefepime (30%), ceftazidime (36%), ceftriaxone (42%), cefotaxime (47%). the ratio of isolates was detected as esbls and ampc producers were 34% and 5.2%, respectively. pcr performed on 12 selected isolates via phenotypic tests and the results revealed that among 12 isolates, 11 contained bla cmy-42 . conclusion : unfortunately, antibiotic resistance has become an increasingly critical problem in many countries like iran and occurrence of isolates co-expressing ampc-β-lactamases and esbls can create serious problems in the future. as antibiotic options in the treatment of ampc β-lactamases and esbls producing organisms are extremely limited, molecular screening by laboratories is suggested to reduce the risk of therapeutic defeat.
منابع مشابه
Detection of plasmid-mediated AmpC beta-lactamase genes in clinical isolates by using multiplex PCR.
Therapeutic options for infections caused by gram-negative organisms expressing plasmid-mediated AmpC beta-lactamases are limited because these organisms are usually resistant to all the beta-lactam antibiotics, except for cefepime, cefpirome, and the carbapenems. These organisms are a major concern in nosocomial infections and should therefore be monitored in surveillance studies. Six families...
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background : ampc beta lactamases play a significant role in creating resistance to third generation cephalosporins worldwide. they mostly express on chromosome of enterobacteriaceae especially escherichia coli and cause consequential problem in clinical treatment and lead to failure in diagnosis and phenotypic test recommended by clinical and laboratory standards institute. methods : total...
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A multiplex, real-time TaqMan assay was designed to identify clinical isolates carrying plasmid-mediated ampC genes. The specificity and sensitivity of this assay were 100% when testing characterized AmpC/non-AmpC-producing isolates and randomly selected clinical isolates. This is a rapid assay that can be performed in a clinical microbiology laboratory.
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Objective(s) Extended-spectrum beta-lactamase (ESBL) producing bacteria have an important role in nosocomial infections. Due to the limited availability of information about the molecular epidemiology of ESBL producing bacteria in Mashhad, we decided to investigate about TEM, CTX and SHV ESBLs among urinary Escherichia coli isolates in Mashhad, a city in northeast Iran. Materials and Methods ...
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عنوان ژورنال:
journal of medical bacteriologyجلد ۵، شماره ۵-۶، صفحات ۲۱-۲۸
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